One way that monoclonal antibodies are produced is through the use of hybridoma cell cultures and harvesting as a hybridoma cell culture supernatant. Hybridoma cell cultures are cells that secrete antibodies, and this process is simple for obtaining monoclonal antibodies for immunochemical processes. The supernatants have a concentration that is high enough for the majority of assays.
Collection of Supernatants
During the collection of a cell culture supernatant, the best clinics produce antibodies by allowing the cultures to grow until the end of the hybridomas exponential growth phase. This way, they can gather higher-titer supernatants. The antibody quality is not impacted by the death of some of these cells. These companies can purify antibodies by selectively enriching or specifically extracting antibodies from the cell culture supernatant of a hybridoma cell line for monoclonal antibodies. There are several different purification processes for monoclonal ascites fluid and cell culture supernatant, or polyclonal antiserum.
Purification Process
Protein A/G purification uses bacterial proteins to bind antibodies. This process is based on high affinity to the immunoglobulin Fc domain. This purification removes most of the serum proteins from the raw antiserum, but it doesn’t eradicate the non-specific immunoglobulin. Affinity tag purification isolates a specific protein or a collection of proteins. It uses a reversible interaction between the protein and a chromatographic matrix combined with a specific ligand to separate the proteins. These processes use the cell culture supernatant process to create the antibodies clients need.
